Sumcad sare oo Iibin Kulul Proteinase K CAS 39450-01-6 Tayada La Dammaanad Qaaday
Waxaan u maleyneynaa waxa macaamiishu u maleynayaan, degdegga degdegga ah ee lagu dhaqmo danaha macaamiisha mabda'a aasaasiga ah, u oggolaanaya tayo sare oo aad u wanaagsan, yareynta kharashyada habaynta, kharashyadu waa kuwo macquul ah oo dheeraad ah, waxaan ku guuleysanay macaamiisha cusub iyo kuwii hore taageerada iyo xaqiijinta sumcadda sare ee Iibinta KululProteinase K CAS 39450-01-6Tayada La Dammaanad Qaaday, Annagoo ah khabiir ku takhasusay qaybtan, waxaan ka go'annahay inaan xallinno dhibaato kasta oo ku saabsan ilaalinta heerkulka sare ee isticmaalayaasha.
Waxaan u maleyneynaa waxa macaamiishu u maleynayaan, degdegga degdegga ah ee lagu dhaqmo danaha macaamiisha mabda'a aasaasiga ah, oo u oggolaanaya tayo sare oo aad u wanaagsan, yareynta kharashyada habaynta, kharashyadu waa kuwo macquul ah oo dheeraad ah, waxaan ku guuleysanay taageerada iyo xaqiijinta macaamiisha cusub iyo kuwii horeProteinase Shiinaha K iyo CAS 39450-01-6Waxaa jira qalab wax soo saar iyo farsamayn oo horumarsan iyo shaqaale xirfad leh si loo hubiyo in alaabta ay tayo sare leedahay. Waxaan helnay adeeg heer sare ah oo ka hor iibka, iibinta, iyo iibka kadib si loo hubiyo macaamiisha hubin kara inay sameeyaan dalabaadka. Ilaa hadda xalalkayagu hadda si dhakhso leh oo aad looga jecel yahay Koonfurta Ameerika, Bariga Aasiya, Bariga Dhexe, Afrika, iwm.
| Dareere No.1 ah | |
| Hawlgal gaar ah oo borotiin ah> 800U/mL, fiirsashada waa 20mg/mL, ma jiraan haraaga Nickase | |
| Budada No.2 | |
| Muuqaalka | Budada cad ee lyophilized |
| Nadiifnimo Elektroforetik ah | ≥95% |
| Dhaqdhaqaaqa enzyme-ka | ≥30U/mg |
| Hadhaaga aashitada nukliyeerku | Aan la arki karin |
| Haraaga Deoxyribonuclease | Aan la arki karin |
| Haraaga Ribonuclease | Aan la arki karin |
| Lambarka Gaariga | G1205-10ML |
| Kaydinta | Marka lagu kaydiyo -20 °C, badeecadu waxay sii haynaysaa firfircooni ugu yaraan 1 sano, iyadoo la qaadayo bacaha barafka. |
Borotiinase Kwaa borotiin serine ah oo ka tirsan qoyska subtilisin oo leh firfircooni enzymatic oo hufan iyo gaar ahaansho ballaaran oo substrate ah, kaas oo si doorbid leh u burburiya xidhmooyinka ester iyo peptides-ka ku xiga C-terminus ee amino acids-ka hydrophobic, amino acids-ka ku jira sulfur, iyo xidhmooyinka amino acids-ka udgoon, kuwaas oo inta badan loo isticmaalo in lagu burburiyo borotiinnada si loo soo saaro peptides-ka gaaban.Borotiinase KWaxay leedahay astaan u ah saddex-geesoodka catalytic-ga caadiga ah ee Asp39-His69-Ser224 oo u gaar ah borotiinka serine waxayna leedahay laba goobood oo isku xira Ca2+ oo ku wareegsan xarunta firfircoon si loo kordhiyo xasilloonideeda loona ilaaliyo firfircoonida enzymatic-ka sare iyadoo la raacayo xaalado kala duwan.
1. Qalabka ogaanshaha hidde-sidaha.
2. Qalabka soo saarista RNA iyo DNA-da.
3. Soo saar qaybaha aan borotiinka lahayn ee unugyada oo baabi'iya wasakhda borotiinka (sida diyaarinta tallaallada DNA-da iyo heparin).
4. Diyaarinta DNA-da Kromosomal-ka ee loogu talagalay Koronto-dhaliyaha Wadnaha.
5. Goynta reer galbeedka.
6. Horumarinta iyo soo saarista reagents-ka albumin-ka enzymatic glycated ee ku jira cilmiga ogaanshaha in vitro.
1. 30mg/dhalo 1g/dhalo ama 100g/dhalo
2. 1kgs/bac bir ah
Proteinase K waa protease serine ah oo deggan oo leh gaar ahaansho ballaaran oo substrate ah. Waxay burburisaa borotiinno badan oo ku jira xaaladda asalka ah xitaa marka ay joogaan saabuunta. Laguma dhaqaajiyo ion-yada birta ah, wakiilada chelating (tusaale ahaan, EDTA), reagents-ka sulfhydryl, ama tripsin ama chymotrypsin inhibitors. Waa mid deggan oo ka sarreeya heerka pH ballaaran (4-12.5), iyadoo leh firfircooni ugu wanaagsan pH 6.5-9.5. Hawlaha waxaa lagu kicin karaa iyadoo lagu daro wakiilada denature (SDS iyo Urea). Denature degdeg ah ee ensaymka waxay dhacdaa heerkulka ka sarreeya 70°C. Autolysis ee ensaymka ayaa si isa soo taraysa u dhacda pH-ka alkaline. Si kastaba ha ahaatee, Proteinase K si buuxda uma hawlgasho autolysis. Qaybo ka mid ah jajabyada ensaymka ayaa sii wada inay ilaaliyaan dhaqdhaqaaqooda proteolytic oo dhammaystiran, xitaa ka dib autolysis ballaaran. Proteinase K waxaa si joogto ah loogu isticmaalaa codsiyada bayoolojiga molecular-ka si loo dheefshiido borotiinnada aan loo baahnayn, sida nucleases-ka DNA-da ama diyaarinta RNA ee ka imanaya noolaha yaryar, unugyada la beeray, iyo dhirta. Ensaymka waxaa caadi ahaan loo isticmaalaa 50–200 ug/ml diyaarinta aashitada nukliyeer-ka ee pH 7.5–8.0 iyo 37~55oC. Waqtiyada ku-shubista way kala duwan yihiin 30 daqiiqo ilaa 18 saacadood.
Protease serine ah oo deggan oo aad u falcelinaya; oo loo isticmaalo kala-soocidda borotiinka iyo aashitada nucleic-ga. Proteinase K waxaa loo isticmaalaa nadiifinta RNA iyo DNA-da unugyada ama khadadka unugyada.
Waxaan u maleyneynaa waxa macaamiishu u maleynayaan, degdegga degdegga ah ee lagu dhaqmo danaha macaamiisha mabda'a aasaasiga ah, oo u oggolaanaya tayo sare oo aad u wanaagsan, yareynta kharashyada habaynta, kharashyadu waa kuwo macquul ah oo dheeraad ah, waxaan ku guuleysanay taageerada iyo xaqiijinta macaamiisha cusub iyo kuwii hore ee sumcadda sare leh ee Iibinta Kulul Proteinase K CAS.39450-01-6Tayada La Dammaanad Qaaday, Annagoo ah khabiir ku takhasusay qaybtan, waxaan ka go'annahay inaan xallinno dhibaato kasta oo ku saabsan ilaalinta heerkulka sare ee isticmaalayaasha.
Sumcad sareProteinase Shiinaha K iyo CAS 39450-01-6Waxaa jira qalab wax soo saar iyo farsamayn oo horumarsan iyo shaqaale xirfad leh si loo hubiyo in alaabta ay tayo sare leedahay. Waxaan helnay adeeg heer sare ah oo ka hor iibka, iibinta, iyo iibka kadib si loo hubiyo macaamiisha hubin kara inay sameeyaan dalabaadka. Ilaa hadda xalalkayagu hadda si dhakhso leh oo aad looga jecel yahay Koonfurta Ameerika, Bariga Aasiya, Bariga Dhexe, Afrika, iwm.
