Sumcad Sare Iibinta Barootiinka K CAS 39450-01-6 Tayada La Xaqiijiyay
Waxaan u malaynayaa waxa macaamiishu u maleynayaan, degdegga ah ee degdega ah si ay u dhaqmaan gudaha danaha booska macaamilka ee mabda'a aasaasiga ah, jidaynayey tayo sare leh oo aad u fiican, la yareeyo kharashyada processing, kharashka waa dheeraad ah oo macquul ah, ku guuleystay macaamiisha cusub iyo kuwii hore ee taageerada iyo xaqiijinta Sumcad Sare Iibinta KululProteinase K CAS 39450-01-6Tayada La Dammaanad qaaday, Khabiir ahaan ku takhasusay goobtan, waxaa naga go'an inaan xallino dhibaato kasta oo ilaalinta heerkulka sare ee isticmaalayaasha.
Waxaan u malaynayaa waxa macaamiishu u maleynayaan, degdegga ah ee degdega ah si ay u dhaqmaan gudaha danaha booska macaamilka ee mabda'a aasaasiga ah, jidaynayey tayo sare leh oo aad u fiican, la yareeyo kharashyada processing, kharashka waa dheeraad ah oo macquul ah, ku guuleystay macaamiisha cusub iyo kuwii hore ee taageerada iyo xaqiijintaShiinaha Proteinase K iyo CAS 39450-01-6, Waxaa jira qalab wax-soo-saar heersare ah & qalabaynta iyo shaqaale xirfad leh si loo hubiyo in alaabta tayada sare leh. Waxaan helnay iib ka hor oo aad u wanaagsan, iibka, iibka ka dib si loo hubiyo macaamiisha in ay ku nastaan in ay sameeyaan amarada. Ilaa hadda xalalkayagu hadda si xawli ah ayey ugu socdaan oo aad caan uga ah Koonfurta Ameerika, Bariga Aasiya, Bariga Dhexe, Afrika, iwm.
| No.1 dareere | |
| Waxqabadka gaarka ah ee borotiinka>800U/ml, xooga saaridu waa 20mg/mL, ma jiro hadhaaga Nickase | |
| No.2 budo | |
| Muuqashada | Budada lyophilized cad |
| Nadiifnimada Electrophoretic | ≥95% |
| Dhaqdhaqaaqa enzyme | ≥30U/mg |
| Haraaga aashitada nucleic | Aan la arki karin |
| Hadhaaga Deoxyribonuclease | Aan la arki karin |
| Haraaga Ribonuclease | Aan la arki karin |
| Gaariga No. | G1205-10ML |
| Kaydinta | Marka lagu kaydiyo -20 °C, alaabtu waxay sii haynaysaa dhaqdhaqaaqa ugu yaraan 1 sano, gaadiidka bacda. |
Proteinase Kwaa serine protease ka tirsan qoyska subtilisin oo leh firfircooni firfircoon oo enzymatic ah iyo gaar ahaan substrate-ka ballaadhan, kaas oo doorbidaya kala-goynta ester bonds iyo peptides ee u dhow C-terminus ee acids amino acids, sulfur-ka kooban amino acids, iyo bonds amino acids udgoon, kuwaas oo ah inta badan loo isticmaalo in lagu dhimo borotiinka si loo soo saaro peptides gaaban.Proteinase Kwaxay leedahay sifada triad catalytic-ka caadiga ah Asp39-His69-Ser224 oo u gaar ah proteases serine waxayna leedahay laba goobood oo Ca2+ ah oo ku wareegsan xarunta firfircoon si ay u kordhiso xasiloonideeda oo ay u ilaaliso dhaqdhaqaaqa enzymatic sare ee hoos yimaada xaalado kala duwan.
1. Xirmada ogaanshaha hidda-socodka.
2. Qalabka soo saarista RNA iyo DNA.
3. Ka soo saar qaybaha aan borotiinka ahayn ee unugyada waxayna hoos u dhigaan wasakhda borotiinka (sida diyaarinta tallaalada DNA-da iyo heparin).
4. Diyaarinta Chromosomal DNA-da ee Pulse Electrophoresis.
5. Burburinta reer galbeedka.
6. Horumarinta iyo soo saarista enzymatic glycated albumin reagents ee berrinkii ogaanshaha in vitro.
1. 30mg/dhalo 1g/dhalo ama 100g/dhalo
2. 1kgs/bacda biilka
Proteinase K waa serine protease deggan oo leh substrate gaar ah. Waxay hoos u dhigtaa borotiinno badan oo ku jira gobolka hooyo xitaa marka ay joogaan saabuunta. Laguma hawlgeliyo ion bir ah, wakiilada chelating (tusaale, EDTA), sulfhydryl reagents, ama tripsin ama chymotrypsin inhibitors. Waxay ku xasilloon tahay pH ballaaran oo kala duwan (4-12.5), oo leh firfircooni ugu fiican pH 6.5-9.5. Dhaqdhaqaaqa waxa lagu kicin karaa marka lagu daro wakiilada ilkaha (SDS iyo Urea). Denaturation-ka degdega ah ee ensaymku wuxuu ku dhacaa heerkul ka sarreeya 70 ° C. Autolysis of ensaymku waxay ku sii kordheysaa pH alkaline. Si kastaba ha ahaatee, Proteinase K si buuxda uma hawlgelin autolysis. Qaar ka mid ah jajabyada enzymes waxay sii wadaan inay sii wadaan dhaqdhaqaaqooda borotiinka oo dhammaystiran, xitaa ka dib markii si ballaaran loo sameeyo. Proteinase K waxaa inta badan loo isticmaalaa codsiyada bayooloojiyada molecular si loo dheefshiido borotiinnada aan loo baahnayn, sida nucleasesyada DNA ama diyaarinta RNA ee unugyada yaryar, unugyada dhaqanka, iyo dhirta. Enzyme waxaa sida caadiga ah loo isticmaalaa 50-200 ug/ml ee diyaarinta nucleic acid ee pH 7.5-8.0 iyo 37 ~ 55oC. Waqtiyada soo-kabashada waxay ku kala duwan yihiin 30 daqiiqo ilaa 18 saacadood.
Serine protease deggan oo aad u firfircoon; loo isticmaalo go'doominta borotiinka iyo aashitada nukliyeerka.Proteinase K waxaa loo isticmaalaa sifaynta RNA iyo DNA ee unugyada ama xariiqyada unugyada.
Waxaan u malaynayaa waxa macaamiishu u maleynayaan, degdegga ah ee degdega ah si ay u dhaqmaan gudaha danaha booska macaamilka ee mabda'a aasaasiga ah, jidaynayey tayo sare leh oo aad u fiican, la yareeyo kharashyada processing, kharashka waa dheeraad ah oo macquul ah, ku guuleystay macaamiisha cusub iyo kuwii hore ee taageerada iyo xaqiijinta Sumcadda Sare ee iibinta Kulul Proteinase K CAS39450-01-6Tayada La Dammaanad qaaday, Khabiir ahaan ku takhasusay goobtan, waxaa naga go'an inaan xallino dhibaato kasta oo ilaalinta heerkulka sare ee isticmaalayaasha.
Sumcad sareShiinaha Proteinase K iyo CAS 39450-01-6, Waxaa jira qalab wax-soo-saar heersare ah & qalabaynta iyo shaqaale xirfad leh si loo hubiyo in alaabta tayada sare leh. Waxaan helnay iib ka hor oo aad u wanaagsan, iibka, iibka ka dib si loo hubiyo macaamiisha in ay ku nastaan in ay sameeyaan amarada. Ilaa hadda xalalkayagu hadda si xawli ah ayey ugu socdaan oo aad caan uga ah Koonfurta Ameerika, Bariga Aasiya, Bariga Dhexe, Afrika, iwm.
