Warshada ODM Proteinase K CAS No. 39450-01-6 ee Horumarinta Awooda Lafaha
Komishankeena waa in uu u adeego isticmaalayaashayada iyo macaamiisheena tayada ugu wanaagsan iyo tartanka la qaadi karo ee alaabta dhijitaalka ah ee ODM Factory Proteinase K CAS No. 39450-01-6 ee Horumarinta Awooda Lafaha xalal tayo leh iyo gaarsiin degdeg ah. Waxaan si dhab ah u rajaynaynaa inaad na siin karto fursad aan ku siino oo aad noqoto lamaanahaaga ugu fiican!
Komishankeenu waa in aanu ugu adeegno isticmaalayaashayada iyo macaamiisheena tayada ugu wanaagsan iyo tartanka la qaadi karo ee alaabta dhijitaalka ahWax soo saarka Kiimikada Shiinaha iyo Alaabta ceeriin, Waxay yihiin qaabaynta adag oo kor u qaadaya si wax ku ool ah aduunka oo dhan. Weligaa ha ku lumin hawlihii waaweynaa waqti degdeg ah gudaheed, adiga ayay shakhsi ahaan kuu tahay tayada wanaagsan ee cajiibka ah. Waxaa haga mabda'a taxaddar, hufnaan, midnimo iyo hal-abuurnimo. shirkadda. dadaal aad u wanaagsan si aad u balaadhiso ganacsigeeda caalamiga ah, kor u qaad ururkeeda. rofid oo kor u qaad miisaankeeda dhoofinta. Waxaan kalsooni ku qabnay in aan yeelan doono rajo ifaysa oo aan dunida oo dhan ku baahin doono sanadaha soo socda.
| No.1 dareere | |
| Waxqabadka gaarka ah ee borotiinka>800U/ml, xooga saaridu waa 20mg/mL, ma jiro hadhaaga Nickase | |
| No.2 budo | |
| Muuqashada | Budada lyophilized cad |
| Nadiifnimada Electrophoretic | ≥95% |
| Dhaqdhaqaaqa enzyme | ≥30U/mg |
| Haraaga aashitada nucleic | Aan la arki karin |
| Hadhaaga Deoxyribonuclease | Aan la arki karin |
| Haraaga Ribonuclease | Aan la arki karin |
| Gaariga No. | G1205-10ML |
| Kaydinta | Marka lagu kaydiyo -20 °C, alaabtu waxay sii haynaysaa dhaqdhaqaaqa ugu yaraan 1 sano, gaadiidka bacda. |
Proteinase K waa serine protease ka tirsan qoyska subtilisin oo leh firfircooni firfircoon oo enzymatic ah iyo gaar ahaan substrate-ka ballaaran, kaas oo doorbidaya kala jajabiya curaarta esterka iyo peptides ee u dhow C-terminus ee acids amino acids, sulfur-ka kooban amino acids, iyo bonds amino acids udgoon. kuwaas oo inta badan loo isticmaalo hoos u dhigista borotiinka si loo soo saaro peptides gaaban. Proteinase K waxay leedahay sifada triad catalytic-ka caadiga ah Asp39-His69-Ser224 oo u gaar ah proteases serine waxayna leedahay laba goobood oo Ca2+ ah oo ku wareegsan xarunta firfircoon si ay u kordhiso xasilloonideeda oo ay u ilaaliso dhaqdhaqaaqa enzymatic sare ee hoos yimaada xaalado kala duwan.
1. Xirmada ogaanshaha hidda-socodka.
2. Qalabka soo saarista RNA iyo DNA.
3. Ka soo saar qaybaha aan borotiinka ahayn ee unugyada waxayna hoos u dhigaan wasakhda borotiinka (sida diyaarinta tallaalada DNA-da iyo heparin).
4. Diyaarinta Chromosomal DNA-da ee Pulse Electrophoresis.
5. Burburinta reer galbeedka.
6. Horumarinta iyo soo saarista enzymatic glycated albumin reagents ee berrinkii ogaanshaha in vitro.
1. 30mg/dhalo 1g/dhalo ama 100g/dhalo
2. 1kgs/bacda biilka
Proteinase K waa serine protease deggan oo leh substrate gaar ah. Waxay hoos u dhigtaa borotiinno badan oo ku jira gobolka hooyo xitaa marka ay joogaan saabuunta. Laguma hawlgeliyo ion bir ah, wakiilada chelating (tusaale, EDTA), sulfhydryl reagents, ama tripsin ama chymotrypsin inhibitors. Waxay ku xasilloon tahay pH ballaaran oo kala duwan (4-12.5), oo leh firfircooni ugu fiican pH 6.5-9.5. Dhaqdhaqaaqa waxa lagu kicin karaa marka lagu daro wakiilada ilkaha (SDS iyo Urea). Denaturation-ka degdega ah ee ensaymku wuxuu ku dhacaa heerkul ka sarreeya 70 ° C. Autolysis of ensaymku waxay ku sii kordheysaa pH alkaline. Si kastaba ha ahaatee, Proteinase K si buuxda uma hawlgelin autolysis. Qaar ka mid ah jajabyada enzymes waxay sii wadaan inay sii wadaan dhaqdhaqaaqooda borotiinka oo dhammaystiran, xitaa ka dib markii si ballaaran loo sameeyo. Proteinase K waxaa inta badan loo isticmaalaa codsiyada bayooloojiyada molecular si loo dheefshiido borotiinnada aan loo baahnayn, sida nucleasesyada DNA ama diyaarinta RNA ee unugyada yaryar, unugyada dhaqanka, iyo dhirta. Enzyme waxaa sida caadiga ah loo isticmaalaa 50-200 ug/ml ee diyaarinta nucleic acid ee pH 7.5-8.0 iyo 37 ~ 55oC. Waqtiyada soo-kabashada waxay ku kala duwan yihiin 30 daqiiqo ilaa 18 saacadood.
Serine protease deggan oo aad u firfircoon; loo isticmaalo go'doominta borotiinka iyo aashitada nukliyeerka.Proteinase K waxaa loo isticmaalaa sifaynta RNA iyo DNA ee unugyada ama xariiqyada unugyada.
Komishankeena waa in uu u adeego isticmaalayaashayada iyo macaamiisheena tayada ugu wanaagsan iyo tartanka la qaadi karo ee alaabta dhijitaalka ah ee ODM Factory Proteinase K CAS No. 39450-01-6 ee Horumarinta Awooda Lafaha xalal tayo leh iyo gaarsiin degdeg ah. Waxaan si dhab ah u rajaynaynaa inaad na siin karto fursad aan ku siino oo aad noqoto lamaanahaaga ugu fiican!
Warshada ODMWax soo saarka Kiimikada Shiinaha iyo Alaabta ceeriin, Waxay yihiin qaabaynta adag oo kor u qaadaya si wax ku ool ah aduunka oo dhan. Weligaa ha ku lumin hawlihii waaweynaa waqti degdeg ah gudaheed, adiga ayay shakhsi ahaan kuu tahay tayada wanaagsan ee cajiibka ah. Waxaa haga mabda'a taxaddar, hufnaan, midnimo iyo hal-abuurnimo. shirkadda. dadaal aad u wanaagsan si aad u balaadhiso ganacsigeeda caalamiga ah, kor u qaad ururkeeda. rofid oo kor u qaad miisaankeeda dhoofinta. Waxaan kalsooni ku qabnay in aan yeelan doono rajo ifaysa oo aan dunida oo dhan ku baahin doono sanadaha soo socda.
