ODM Warshadda Proteinase K CAS No. 39450-01-6 si loo Hagaajiyo Xoogga Lafaha
Guddigayagu waa inaan u adeegno isticmaalayaashayada iyo macaamiisheenna alaabada dijitaalka ah ee la qaadi karo ee ugu tayada wanaagsan uguna tartan badan ee ODM Factory Proteinase K CAS No. 39450-01-6 si loo Hagaajiyo Xoogga Lafaha, Waxaan dareemeynaa inaad ku farxi doonto qiimaha iibka ee la awoodi karo, xalalka tayada sare leh iyo gaarsiinta degdegga ah. Waxaan si daacad ah u rajeyneynaa inaad na siin karto fursad aan ku siino oo aan noqono lammaanahaaga ugu fiican!
Guddigayagu waa inaan u adeegno isticmaalayaashayada iyo macaamiisheenna alaabada dijitaalka ah ee la qaadi karo ee ugu tayada wanaagsan uguna tartan badanBadeecadaha Kiimikada Shiinaha iyo Agabka CeeriinWaa qaab-dhismeed adag oo si wax ku ool ah u kobcinaya adduunka oo dhan. Weligeed ma baabi'inayaan hawlaha waaweyn waqti yar gudaheed, waa waajib adiga shaqsi ahaan aad u fiican oo tayo wanaagsan leh. Iyada oo ay hagayso mabda'a Feejignaan, Hufnaan, Midnimo iyo Hal-abuur. shirkaddu waxay ku dadaalaysaa inay ballaariso ganacsigeeda caalamiga ah, kor u qaaddo ururkeeda, kobciso oo ay kor u qaaddo heerka dhoofinta. Waxaan ku kalsoonnahay inaan yeelan doonno rajo wanaagsan oo aan ku faafin doonno adduunka oo dhan sannadaha soo socda.
| Dareere No.1 ah | |
| Hawlgal gaar ah oo borotiin ah> 800U/mL, fiirsashada waa 20mg/mL, ma jiraan haraaga Nickase | |
| Budada No.2 | |
| Muuqaalka | Budada cad ee lyophilized |
| Nadiifnimo Elektroforetik ah | ≥95% |
| Dhaqdhaqaaqa enzyme-ka | ≥30U/mg |
| Hadhaaga aashitada nukliyeerku | Aan la arki karin |
| Haraaga Deoxyribonuclease | Aan la arki karin |
| Haraaga Ribonuclease | Aan la arki karin |
| Lambarka Gaariga | G1205-10ML |
| Kaydinta | Marka lagu kaydiyo -20 °C, badeecadu waxay sii haynaysaa firfircooni ugu yaraan 1 sano, iyadoo la qaadayo bacaha barafka. |
Proteinase K waa borotiin serine ah oo ka tirsan qoyska subtilisin oo leh firfircooni enzymatic oo hufan iyo gaar ahaansho ballaaran oo substrate ah, kaas oo si doorbid leh u burburiya xidhmooyinka ester iyo peptides ee ku xiga C-terminus ee amino acids hydrophobic, amino acids-ka ku jira sulfur, iyo xidhmooyinka amino acids aromatic, kuwaas oo inta badan loo isticmaalo in lagu burburiyo borotiinnada si loo soo saaro peptides gaagaaban. Proteinase K waxay leedahay astaamaha saddex-geesoodka catalytic ee caadiga ah Asp39-His69-Ser224 oo u gaar ah proteinases-ka serine waxayna leedahay laba goobood oo isku xidha Ca2+ oo ku wareegsan xarunta firfircoon si loo kordhiyo xasilloonideeda loona ilaaliyo firfircoonida enzymatic sare iyadoo la raacayo xaalado kala duwan.
1. Qalabka ogaanshaha hidde-sidaha.
2. Qalabka soo saarista RNA iyo DNA-da.
3. Soo saar qaybaha aan borotiinka lahayn ee unugyada oo baabi'iya wasakhda borotiinka (sida diyaarinta tallaallada DNA-da iyo heparin).
4. Diyaarinta DNA-da Kromosomal-ka ee loogu talagalay Koronto-dhaliyaha Wadnaha.
5. Goynta reer galbeedka.
6. Horumarinta iyo soo saarista reagents-ka albumin-ka enzymatic glycated ee ku jira cilmiga ogaanshaha in vitro.
1. 30mg/dhalo 1g/dhalo ama 100g/dhalo
2. 1kgs/bac bir ah
Proteinase K waa protease serine ah oo deggan oo leh gaar ahaansho ballaaran oo substrate ah. Waxay burburisaa borotiinno badan oo ku jira xaaladda asalka ah xitaa marka ay joogaan saabuunta. Laguma dhaqaajiyo ion-yada birta ah, wakiilada chelating (tusaale ahaan, EDTA), reagents-ka sulfhydryl, ama tripsin ama chymotrypsin inhibitors. Waa mid deggan oo ka sarreeya heerka pH ballaaran (4-12.5), iyadoo leh firfircooni ugu wanaagsan pH 6.5-9.5. Hawlaha waxaa lagu kicin karaa iyadoo lagu daro wakiilada denature (SDS iyo Urea). Denature degdeg ah ee ensaymka waxay dhacdaa heerkulka ka sarreeya 70°C. Autolysis ee ensaymka ayaa si isa soo taraysa u dhacda pH-ka alkaline. Si kastaba ha ahaatee, Proteinase K si buuxda uma hawlgasho autolysis. Qaybo ka mid ah jajabyada ensaymka ayaa sii wada inay ilaaliyaan dhaqdhaqaaqooda proteolytic oo dhammaystiran, xitaa ka dib autolysis ballaaran. Proteinase K waxaa si joogto ah loogu isticmaalaa codsiyada bayoolojiga molecular-ka si loo dheefshiido borotiinnada aan loo baahnayn, sida nucleases-ka DNA-da ama diyaarinta RNA ee ka imanaya noolaha yaryar, unugyada la beeray, iyo dhirta. Ensaymka waxaa caadi ahaan loo isticmaalaa 50–200 ug/ml diyaarinta aashitada nukliyeer-ka ee pH 7.5–8.0 iyo 37~55oC. Waqtiyada ku-shubista way kala duwan yihiin 30 daqiiqo ilaa 18 saacadood.
Protease serine ah oo deggan oo aad u falcelinaya; oo loo isticmaalo kala-soocidda borotiinka iyo aashitada nucleic-ga. Proteinase K waxaa loo isticmaalaa nadiifinta RNA iyo DNA-da unugyada ama khadadka unugyada.
Guddigayagu waa inaan u adeegno isticmaalayaashayada iyo macaamiisheenna alaabada dijitaalka ah ee la qaadi karo ee ugu tayada wanaagsan uguna tartan badan ee ODM Factory Proteinase K CAS No. 39450-01-6 si loo Hagaajiyo Xoogga Lafaha, Waxaan dareemeynaa inaad ku farxi doonto qiimaha iibka ee la awoodi karo, xalalka tayada sare leh iyo gaarsiinta degdegga ah. Waxaan si daacad ah u rajeyneynaa inaad na siin karto fursad aan ku siino oo aan noqono lammaanahaaga ugu fiican!
Warshadda ODMBadeecadaha Kiimikada Shiinaha iyo Agabka CeeriinWaa qaab-dhismeed adag oo si wax ku ool ah u kobcinaya adduunka oo dhan. Weligeed ma baabi'inayaan hawlaha waaweyn waqti yar gudaheed, waa waajib adiga shaqsi ahaan aad u fiican oo tayo wanaagsan leh. Iyada oo ay hagayso mabda'a Feejignaan, Hufnaan, Midnimo iyo Hal-abuur. shirkaddu waxay ku dadaalaysaa inay ballaariso ganacsigeeda caalamiga ah, kor u qaaddo ururkeeda, kobciso oo ay kor u qaaddo heerka dhoofinta. Waxaan ku kalsoonnahay inaan yeelan doonno rajo wanaagsan oo aan ku faafin doonno adduunka oo dhan sannadaha soo socda.
