Naqshadaynta Xirfadlaha Boroteinase Tayo Sare leh K CAS No. 39450-01-6 oo leh Qiimaha ugu Wanaagsan
Caadi ahaan waxaanu qabanaa inaanu ahaano shaqaale la taaban karo anagoo hubinayna inaan ku siin doono faa'iidada ugu fiican iyo qiimaha iibinta ugu wanaagsan ee Nakhshad Xirfadeed Tayada SareProteinase K CAS No. 39450-01-6leh Qiimaha ugu Wanaagsan, Wixii macluumaad dheeraad ah iyo xaqiiqooyinka, fadlan nala hadal sida ugu dhakhsaha badan ee suurtogalka ah!
Caadi ahaan waxaanu qabanaa inaanu ahaano shaqaale la taaban karo anagoo hubinayna inaan ku siin doono faa'iidada ugu fiican iyo qiimaha ugu iibinta wanaagsanShiinaha Tritirachium Album Limber iyo Molecular Biology, Xakamaynta tayada adag ayaa lagu fuliyay xiriir kasta oo ka mid ah geeddi-socodka wax-soo-saarka oo dhan. Waxaan si dhab ah u rajaynaynaa inaan aasaasno iskaashiga saaxiibtinimo iyo faa'iido-wadaaga adiga kula leh. Iyada oo ku saleysan xalal tayo sare leh iyo adeeg iibka ka dib oo kaamil ah waa fikradeena, macaamiisha qaar ayaa nala shaqeynayay in ka badan 5 sano.
| No.1 dareere | |
| Waxqabadka gaarka ah ee borotiinka>800U/ml, xooga saaridu waa 20mg/mL, ma jiro hadhaaga Nickase | |
| No.2 budo | |
| Muuqashada | Budada lyophilized cad |
| Nadiifnimada Electrophoretic | ≥95% |
| Dhaqdhaqaaqa enzyme | ≥30U/mg |
| Haraaga aashitada nucleic | Aan la arki karin |
| Hadhaaga Deoxyribonuclease | Aan la arki karin |
| Haraaga Ribonuclease | Aan la arki karin |
| Gaariga No. | G1205-10ML |
| Kaydinta | Marka lagu kaydiyo -20 °C, alaabtu waxay sii haynaysaa dhaqdhaqaaqa ugu yaraan 1 sano, gaadiidka bacda. |
Proteinase Kwaa serine protease ka tirsan qoyska subtilisin oo leh firfircooni firfircoon oo enzymatic ah iyo gaar ahaan substrate-ka ballaadhan, kaas oo doorbidaya kala-goynta ester bonds iyo peptides ee u dhow C-terminus ee acids amino acids, sulfur-ka kooban amino acids, iyo bonds amino acids udgoon, kuwaas oo ah inta badan loo isticmaalo in lagu dhimo borotiinka si loo soo saaro peptides gaaban. Proteinase K waxay leedahay sifada triad catalytic-ka caadiga ah Asp39-His69-Ser224 oo u gaar ah proteases serine waxayna leedahay laba goobood oo Ca2+ ah oo ku wareegsan xarunta firfircoon si ay u kordhiso xasilloonideeda oo ay u ilaaliso dhaqdhaqaaqa enzymatic sare ee hoos yimaada xaalado kala duwan.
1. Xirmada ogaanshaha hidda-socodka.
2. Qalabka soo saarista RNA iyo DNA.
3. Ka soo saar qaybaha aan borotiinka ahayn ee unugyada waxayna hoos u dhigaan wasakhda borotiinka (sida diyaarinta tallaalada DNA-da iyo heparin).
4. Diyaarinta Chromosomal DNA-da ee Pulse Electrophoresis.
5. Burburinta reer galbeedka.
6. Horumarinta iyo soo saarista enzymatic glycated albumin reagents ee berrinkii ogaanshaha in vitro.
1. 30mg/dhalo 1g/dhalo ama 100g/dhalo
2. 1kgs/bacda biilka
Proteinase K waa serine protease deggan oo leh substrate gaar ah. Waxay hoos u dhigtaa borotiinno badan oo ku jira gobolka hooyo xitaa marka ay joogaan saabuunta. Laguma hawlgeliyo ion bir ah, wakiilada chelating (tusaale, EDTA), sulfhydryl reagents, ama tripsin ama chymotrypsin inhibitors. Waxay ku xasilloon tahay pH ballaaran oo kala duwan (4-12.5), oo leh firfircooni ugu fiican pH 6.5-9.5. Dhaqdhaqaaqa waxa lagu kicin karaa marka lagu daro wakiilada ilkaha (SDS iyo Urea). Denaturation-ka degdega ah ee ensaymku wuxuu ku dhacaa heerkul ka sarreeya 70 ° C. Autolysis of ensaymku waxay ku sii kordheysaa pH alkaline. Si kastaba ha ahaatee, Proteinase K si buuxda uma hawlgelin autolysis. Qaar ka mid ah jajabyada enzymes waxay sii wadaan inay sii wadaan dhaqdhaqaaqooda borotiinka oo dhammaystiran, xitaa ka dib markii si ballaaran loo sameeyo. Proteinase K waxaa inta badan loo isticmaalaa codsiyada bayooloojiyada molecular si loo dheefshiido borotiinnada aan loo baahnayn, sida nucleasesyada DNA ama diyaarinta RNA ee unugyada yaryar, unugyada dhaqanka, iyo dhirta. Enzyme waxaa sida caadiga ah loo isticmaalaa 50-200 ug/ml ee diyaarinta nucleic acid ee pH 7.5-8.0 iyo 37 ~ 55oC. Waqtiyada soo-kabashada waxay ku kala duwan yihiin 30 daqiiqo ilaa 18 saacadood.
Serine protease deggan oo aad u firfircoon; loo isticmaalo go'doominta borotiinka iyo aashitada nukliyeerka.Proteinase K waxaa loo isticmaalaa sifaynta RNA iyo DNA ee unugyada ama xariiqyada unugyada.
Caadi ahaan waxaanu qabanaa inaanu ahaano shaqaale la taaban karo anagoo hubinayna inaan ku siin doono faa'iidada ugu fiican iyo qiimaha iibinta ugu wanaagsan ee Naqshadeynta Xirfadlayaasha Proteinase Tayada Sare ee K CAS No. 39450-01-6 oo leh Qiimaha ugu Wanaagsan, Wixii macluumaad dheeraad ah iyo xaqiiqooyinka, fadlan ku hadal noo sida ugu dhakhsaha badan ee suurtogalka ah!
Naqshad XirfadeedShiinaha Tritirachium Album Limber iyo Molecular Biology, Xakamaynta tayada adag ayaa lagu fuliyay xiriir kasta oo ka mid ah geeddi-socodka wax-soo-saarka oo dhan. Waxaan si dhab ah u rajaynaynaa inaan aasaasno iskaashiga saaxiibtinimo iyo faa'iido-wadaaga adiga kula leh. Iyada oo ku saleysan xalal tayo sare leh iyo adeeg iibka ka dib oo kaamil ah waa fikradeena, macaamiisha qaar ayaa nala shaqeynayay in ka badan 5 sano.
