Naqshad Xirfadeed Proteinase K CAS No. 39450-01-6 oo leh Qiimaha ugu Fiican
Badanaa waxaan u shaqeynaa sidii shaqaale la taaban karo annagoo hubinayna inaan ku siin doono qiimaha ugu fiican iyo qiimaha ugu wanaagsan ee lagu iibiyo Naqshadeynta Xirfadeed Tayo Sare lehBorotiinase K Lambarka CAS: 39450-01-6Qiimaha ugu Fiican, Wixii macluumaad dheeraad ah iyo xaqiiqooyin ah, fadlan nala soo hadal sida ugu dhakhsaha badan!
Badanaa waxaan u shaqeynaa sidii shaqaale la taaban karo annagoo hubinayna inaan ku siin doono qiimaha ugu fiican iyo kan ugu wanaagsan ee lagu iibiyoBayoolajiga Limber iyo Molecular-ka ee Shiinaha Tritirachium AlbumXakamaynta tayada adag ayaa lagu fuliyaa xiriir kasta oo ka mid ah geeddi-socodka wax soo saarka oo dhan. Waxaan si daacad ah u rajeyneynaa inaan idinla yeelano iskaashi saaxiibtinimo iyo faa'iido leh. Iyada oo ku saleysan xalal tayo sare leh iyo adeeg iib kahor/iibinta kadib oo qumman ayaa fikraddeenna ah, macaamiisha qaar ayaa nala shaqeynayay in ka badan 5 sano.
| Dareere No.1 ah | |
| Hawlgal gaar ah oo borotiin ah> 800U/mL, fiirsashada waa 20mg/mL, ma jiraan haraaga Nickase | |
| Budada No.2 | |
| Muuqaalka | Budada cad ee lyophilized |
| Nadiifnimo Elektroforetik ah | ≥95% |
| Dhaqdhaqaaqa enzyme-ka | ≥30U/mg |
| Hadhaaga aashitada nukliyeerku | Aan la arki karin |
| Haraaga Deoxyribonuclease | Aan la arki karin |
| Haraaga Ribonuclease | Aan la arki karin |
| Lambarka Gaariga | G1205-10ML |
| Kaydinta | Marka lagu kaydiyo -20 °C, badeecadu waxay sii haynaysaa firfircooni ugu yaraan 1 sano, iyadoo la qaadayo bacaha barafka. |
Borotiinase Kwaa borotiin serine ah oo ka tirsan qoyska subtilisin oo leh firfircooni enzymatic oo hufan iyo gaar ahaansho ballaaran oo substrate ah, kaas oo si doorbid leh u burburiya xidhmooyinka ester iyo peptides-ka ku xiga C-terminus ee amino acids-ka hydrophobic, amino acids-ka ku jira sulfur, iyo xidhmooyinka amino acids-ka udgoon, kuwaas oo inta badan loo isticmaalo in lagu burburiyo borotiinnada si loo soo saaro peptides-ka gaaban.Borotiinase KWaxay leedahay astaan u ah saddex-geesoodka catalytic-ga caadiga ah ee Asp39-His69-Ser224 oo u gaar ah borotiinka serine waxayna leedahay laba goobood oo isku xira Ca2+ oo ku wareegsan xarunta firfircoon si loo kordhiyo xasilloonideeda loona ilaaliyo firfircoonida enzymatic-ka sare iyadoo la raacayo xaalado kala duwan.
1. Qalabka ogaanshaha hidde-sidaha.
2. Qalabka soo saarista RNA iyo DNA-da.
3. Soo saar qaybaha aan borotiinka lahayn ee unugyada oo baabi'iya wasakhda borotiinka (sida diyaarinta tallaallada DNA-da iyo heparin).
4. Diyaarinta DNA-da Kromosomal-ka ee loogu talagalay Koronto-dhaliyaha Wadnaha.
5. Goynta reer galbeedka.
6. Horumarinta iyo soo saarista reagents-ka albumin-ka enzymatic glycated ee ku jira cilmiga ogaanshaha in vitro.
1. 30mg/dhalo 1g/dhalo ama 100g/dhalo
2. 1kgs/bac bir ah
Proteinase K waa protease serine ah oo deggan oo leh gaar ahaansho ballaaran oo substrate ah. Waxay burburisaa borotiinno badan oo ku jira xaaladda asalka ah xitaa marka ay joogaan saabuunta. Laguma dhaqaajiyo ion-yada birta ah, wakiilada chelating (tusaale ahaan, EDTA), reagents-ka sulfhydryl, ama tripsin ama chymotrypsin inhibitors. Waa mid deggan oo ka sarreeya heerka pH ballaaran (4-12.5), iyadoo leh firfircooni ugu wanaagsan pH 6.5-9.5. Hawlaha waxaa lagu kicin karaa iyadoo lagu daro wakiilada denature (SDS iyo Urea). Denature degdeg ah ee ensaymka waxay dhacdaa heerkulka ka sarreeya 70°C. Autolysis ee ensaymka ayaa si isa soo taraysa u dhacda pH-ka alkaline. Si kastaba ha ahaatee, Proteinase K si buuxda uma hawlgasho autolysis. Qaybo ka mid ah jajabyada ensaymka ayaa sii wada inay ilaaliyaan dhaqdhaqaaqooda proteolytic oo dhammaystiran, xitaa ka dib autolysis ballaaran. Proteinase K waxaa si joogto ah loogu isticmaalaa codsiyada bayoolojiga molecular-ka si loo dheefshiido borotiinnada aan loo baahnayn, sida nucleases-ka DNA-da ama diyaarinta RNA ee ka imanaya noolaha yaryar, unugyada la beeray, iyo dhirta. Ensaymka waxaa caadi ahaan loo isticmaalaa 50–200 ug/ml diyaarinta aashitada nukliyeer-ka ee pH 7.5–8.0 iyo 37~55oC. Waqtiyada ku-shubista way kala duwan yihiin 30 daqiiqo ilaa 18 saacadood.
Protease serine ah oo deggan oo aad u falcelinaya; oo loo isticmaalo kala-soocidda borotiinka iyo aashitada nucleic-ga. Proteinase K waxaa loo isticmaalaa nadiifinta RNA iyo DNA-da unugyada ama khadadka unugyada.
Badanaa waxaan qabannaa shaqo la taaban karo annagoo hubinayna inaan ku siin doono qiimaha ugu faa'iidada badan iyo qiimaha ugu wanaagsan ee iibka ah ee Naqshadeynta Xirfadeed ee Tayada Sare leh Proteinase K CAS No. 39450-01-6 oo leh Qiimaha ugu Fiican. Wixii macluumaad dheeraad ah iyo xaqiiqooyin, fadlan nala soo xiriir sida ugu dhakhsaha badan!
Naqshad XirfadeedBayoolajiga Limber iyo Molecular-ka ee Shiinaha Tritirachium AlbumXakamaynta tayada adag ayaa lagu fuliyaa xiriir kasta oo ka mid ah geeddi-socodka wax soo saarka oo dhan. Waxaan si daacad ah u rajeyneynaa inaan idinla yeelano iskaashi saaxiibtinimo iyo faa'iido leh. Iyada oo ku saleysan xalal tayo sare leh iyo adeeg iib kahor/iibinta kadib oo qumman ayaa fikraddeenna ah, macaamiisha qaar ayaa nala shaqeynayay in ka badan 5 sano.
