Warshadda ugu iibsiga badan Adduunka oo dhan Proteinase K CAS 39450-01-6 oo leh Heerka Soo-gaadhista Sare iyo Taariikhda Ugu Dambeysay ee Wax-soo-saarka
Xubin kasta oo shaqsi ah oo ka tirsan shaqaalaheenna dakhliga waxqabadka badan ayaa qiimeeya shuruudaha macaamiisha iyo isgaarsiinta ururka ee Warshadda ugu iibinta badan Adduunka oo dhanProteinase K CAS 39450-01-6Iyada oo leh Heerka Soo-gaadhista Sare iyo Taariikhda Ugu Dambeysay ee Wax-soo-saarka, hadda, waxaan rajeyneynaa iskaashi ka sii weyn oo lala yeesho macaamiisha dibadda iyadoo lagu saleynayo faa'iidooyinka labada dhinac. Hubi inaadan dareemin wax kharash ah si aad noola soo xiriirto si aad u hesho macluumaad dheeraad ah.
Xubin kasta oo ka tirsan shaqaalahayaga dakhliga waxqabadka weyn waxay qiimeeyaan shuruudaha macaamiisha iyo isgaarsiinta ururkaShiinaha 39450-01-6Waxaan si diirran u soo dhaweyneynaa taageeradaada waxaanan macaamiisheenna ugu adeegi doonnaa gudaha iyo dibaddaba alaab tayo sare leh iyo adeeg heer sare ah oo loogu talagalay isbeddelka horumarinta dheeraadka ah sida had iyo jeer. Waxaan aaminsanahay inaad dhawaan ka faa'iideysan doonto xirfaddayada.
| Dareere No.1 ah | |
| Hawlgal gaar ah oo borotiin ah> 800U/mL, fiirsashada waa 20mg/mL, ma jiraan haraaga Nickase | |
| Budada No.2 | |
| Muuqaalka | Budada cad ee lyophilized |
| Nadiifnimo Elektroforetik ah | ≥95% |
| Dhaqdhaqaaqa enzyme-ka | ≥30U/mg |
| Hadhaaga aashitada nukliyeerku | Aan la arki karin |
| Haraaga Deoxyribonuclease | Aan la arki karin |
| Haraaga Ribonuclease | Aan la arki karin |
| Lambarka Gaariga | G1205-10ML |
| Kaydinta | Marka lagu kaydiyo -20 °C, badeecadu waxay sii haynaysaa firfircooni ugu yaraan 1 sano, iyadoo la qaadayo bacaha barafka. |
Borotiinase Kwaa borotiin serine ah oo ka tirsan qoyska subtilisin oo leh firfircooni enzymatic oo hufan iyo gaar ahaansho ballaaran oo substrate ah, kaas oo si doorbid leh u burburiya xidhmooyinka ester iyo peptides-ka ku xiga C-terminus ee amino acids-ka hydrophobic, amino acids-ka ku jira sulfur, iyo xidhmooyinka amino acids-ka udgoon, kuwaas oo inta badan loo isticmaalo in lagu burburiyo borotiinnada si loo soo saaro peptides-ka gaaban.Borotiinase KWaxay leedahay astaan u ah saddex-geesoodka catalytic-ga caadiga ah ee Asp39-His69-Ser224 oo u gaar ah borotiinka serine waxayna leedahay laba goobood oo isku xira Ca2+ oo ku wareegsan xarunta firfircoon si loo kordhiyo xasilloonideeda loona ilaaliyo firfircoonida enzymatic-ka sare iyadoo la raacayo xaalado kala duwan.
1. Qalabka ogaanshaha hidde-sidaha.
2. Qalabka soo saarista RNA iyo DNA-da.
3. Soo saar qaybaha aan borotiinka lahayn ee unugyada oo baabi'iya wasakhda borotiinka (sida diyaarinta tallaallada DNA-da iyo heparin).
4. Diyaarinta DNA-da Kromosomal-ka ee loogu talagalay Koronto-dhaliyaha Wadnaha.
5. Goynta reer galbeedka.
6. Horumarinta iyo soo saarista reagents-ka albumin-ka enzymatic glycated ee ku jira cilmiga ogaanshaha in vitro.
1. 30mg/dhalo 1g/dhalo ama 100g/dhalo
2. 1kgs/bac bir ah
Proteinase K waa protease serine ah oo deggan oo leh gaar ahaansho ballaaran oo substrate ah. Waxay burburisaa borotiinno badan oo ku jira xaaladda asalka ah xitaa marka ay joogaan saabuunta. Laguma dhaqaajiyo ion-yada birta ah, wakiilada chelating (tusaale ahaan, EDTA), reagents-ka sulfhydryl, ama tripsin ama chymotrypsin inhibitors. Waa mid deggan oo ka sarreeya heerka pH ballaaran (4-12.5), iyadoo leh firfircooni ugu wanaagsan pH 6.5-9.5. Hawlaha waxaa lagu kicin karaa iyadoo lagu daro wakiilada denature (SDS iyo Urea). Denature degdeg ah ee ensaymka waxay dhacdaa heerkulka ka sarreeya 70°C. Autolysis ee ensaymka ayaa si isa soo taraysa u dhacda pH-ka alkaline. Si kastaba ha ahaatee, Proteinase K si buuxda uma hawlgasho autolysis. Qaybo ka mid ah jajabyada ensaymka ayaa sii wada inay ilaaliyaan dhaqdhaqaaqooda proteolytic oo dhammaystiran, xitaa ka dib autolysis ballaaran. Proteinase K waxaa si joogto ah loogu isticmaalaa codsiyada bayoolojiga molecular-ka si loo dheefshiido borotiinnada aan loo baahnayn, sida nucleases-ka DNA-da ama diyaarinta RNA ee ka imanaya noolaha yaryar, unugyada la beeray, iyo dhirta. Ensaymka waxaa caadi ahaan loo isticmaalaa 50–200 ug/ml diyaarinta aashitada nukliyeer-ka ee pH 7.5–8.0 iyo 37~55oC. Waqtiyada ku-shubista way kala duwan yihiin 30 daqiiqo ilaa 18 saacadood.
Protease serine ah oo deggan oo aad u falcelinaya; oo loo isticmaalo kala-soocidda borotiinka iyo aashitada nucleic-ga. Proteinase K waxaa loo isticmaalaa nadiifinta RNA iyo DNA-da unugyada ama khadadka unugyada.
Xubin kasta oo shaqsi ah oo ka tirsan shaqaalaheenna dakhliga waxqabadka badan ayaa qiimeeya shuruudaha macaamiisha iyo isgaarsiinta ururka ee Warshadda ugu iibinta badan Adduunka ee Proteinase K CAS39450-01-6Iyada oo leh Heerka Soo-gaadhista Sare iyo Taariikhda Ugu Dambeysay ee Wax-soo-saarka, hadda, waxaan rajeyneynaa iskaashi ka sii weyn oo lala yeesho macaamiisha dibadda iyadoo lagu saleynayo faa'iidooyinka labada dhinac. Hubi inaadan dareemin wax kharash ah si aad noola soo xiriirto si aad u hesho macluumaad dheeraad ah.
Warshadda ugu iibsiga badanShiinaha 39450-01-6Waxaan si diiran u soo dhaweyneynaa taageeradaada waxaanan macaamiisheenna ugu adeegi doonnaa gudaha iyo dibaddaba alaab tayo sare leh iyo adeeg heer sare ah oo loogu talagalay isbeddelka horumarinta dheeraadka ah sida had iyo jeer. Waxaan aaminsanahay inaad dhawaan ka faa'iideysan doonto xirfaddayada.
