Warshada ugu iibinta wanaagsan ee Proteinase-ka Caanka ah ee Caalamka K CAS 39450-01-6 oo leh Heer Gaar ah oo Sare iyo Taariikhda Wax-soosaarka
Xubin kasta oo ka mid ah shaqaalaheenna dakhliga waxqabadka ballaaran ayaa qiimeeya shuruudaha macaamiisha iyo xiriirka ururka ee Warshada ugu iibinta wanaagsan CaalamkaProteinase K CAS 39450-01-6Iyada oo leh Heer Sare oo Soo-gaadhis ah iyo Taariikhda Wax-soo-saarka ee ugu Dambeeyay, Hadda, waxaan rajeyneynaa xitaa wadashaqeyn ballaaran oo lala yeesho macaamiisha dibedda ee ku saleysan faa'iidooyinka wadaagga ah.Hubi inaad dareento wax kharash ah oo aad nagala soo xidhiidho wixii macluumaad dheeraad ah.
Xubin kasta oo ka mid ah shaqaalaheenna dakhliga waxqabadka ballaaran waxay qiimeeyaan shuruudaha macaamiisha iyo xiriirka ururka eeShiinaha 39450-01-6, Waxaan si diiran u soo dhaweyneynaa mas'uuliyaddaada waxaana ugu adeegi doonaa macaamiisheena gudaha iyo dibaddaba alaab tayo sare leh iyo adeeg aad u wanaagsan oo loogu talagalay isbeddelka horumarka dheeraadka ah sida had iyo jeer.Waxaan aaminsanahay inaad ka faa'iidaysan doonto xirfadeena dhawaan.
| No.1 dareere | |
| Waxqabadka gaarka ah ee borotiinka>800U/ml, xooga saaridu waa 20mg/mL, ma jiro hadhaaga Nickase | |
| No.2 budo | |
| Muuqashada | Budada lyophilized cad |
| Nadiifnimada Electrophoretic | ≥95% |
| Dhaqdhaqaaqa enzyme | ≥30U/mg |
| Haraaga aashitada nucleic | Aan la arki karin |
| Hadhaaga Deoxyribonuclease | Aan la arki karin |
| Haraaga Ribonuclease | Aan la arki karin |
| Gaariga No. | G1205-10ML |
| Kaydinta | Marka lagu kaydiyo -20 °C, alaabtu waxay sii haynaysaa dhaqdhaqaaqa ugu yaraan 1 sano, gaadiidka bacda. |
Proteinase Kwaa serine protease ka tirsan qoyska subtilisin oo leh firfircooni firfircoon oo enzymatic ah iyo gaar ahaan substrate-ka ballaadhan, kaas oo doorbidaya kala-goynta ester bonds iyo peptides ee u dhow C-terminus ee acids amino acids, sulfur-ka kooban amino acids, iyo bonds amino acids udgoon, kuwaas oo ah inta badan loo isticmaalo in lagu dhimo borotiinka si loo soo saaro peptides gaaban.Proteinase K waxay leedahay sifada triad catalytic ee caadiga ah Asp39-His69-Ser224 oo u gaar ah proteases serine waxayna leedahay laba goobood oo Ca2+ ah oo ku wareegsan xarunta firfircoon si ay u kordhiso xasilloonideeda oo ay u ilaaliso dhaqdhaqaaqa sare ee enzymatic hoos yimaada xaalado kala duwan.
1. Xirmada ogaanshaha hidda-socodka.
2. Qalabka soo saarista RNA iyo DNA.
3. Ka soo saar qaybaha aan borotiinka ahayn ee unugyada waxayna hoos u dhigaan wasakhda borotiinka (sida diyaarinta tallaalada DNA-da iyo heparin).
4. Diyaarinta Chromosomal DNA-da ee Pulse Electrophoresis.
5. Burburinta reer galbeedka.
6. Horumarinta iyo soo saarista enzymatic glycated albumin reagents ee berrinkii ogaanshaha in vitro.
1. 30mg/dhalo 1g/dhalo ama 100g/dhalo
2. 1kgs/bacda biilka
Proteinase K waa serine protease deggan oo leh substrate gaar ah.Waxay hoos u dhigtaa borotiinno badan oo ku jira gobolka hooyo xitaa marka ay joogaan saabuunta.Laguma hawlgeliyo ion bir ah, wakiilada chelating (tusaale, EDTA), sulfhydryl reagents, ama tripsin ama chymotrypsin inhibitors.Waxay ku xasilloon tahay pH ballaaran oo kala duwan (4-12.5), oo leh firfircooni ugu fiican pH 6.5-9.5.Dhaqdhaqaaqa waxa lagu kicin karaa marka lagu daro wakiilada ilkaha (SDS iyo Urea).Denaturation-ka degdega ah ee ensaymku wuxuu ku dhacaa heerkul ka sarreeya 70 ° C.Autolysis of ensaymku waxay ku sii kordheysaa pH alkaline.Si kastaba ha ahaatee, Proteinase K si buuxda uma hawlgelin autolysis.Qaar ka mid ah jajabyada enzymesku waxay sii wadaan inay sii wadaan dhaqdhaqaaqooda borotiinka oo dhammaystiran, xitaa ka dib markii si ballaaran loo sameeyo.Proteinase K waxaa inta badan loo isticmaalaa codsiyada bayooloojiyada molecular si loo dheefshiido borotiinnada aan loo baahnayn, sida nucleasesyada DNA ama diyaarinta RNA ee unugyada yaryar, unugyada dhaqanka, iyo dhirta.Insaymiska waxaa sida caadiga ah loo isticmaalaa 50-200 ug/ml ee diyaarinta nucleic acid at pH 7.5-8.0 iyo 37 ~ 55oC.Waqtiyada soo-kabashada waxay ku kala duwan yihiin 30 daqiiqo ilaa 18 saacadood.
Serine protease deggan oo aad u firfircoon;loo isticmaalo go'doominta borotiinka iyo aashitada nukliyeerka.Proteinase K waxaa loo isticmaalaa sifaynta RNA iyo DNA ee unugyada ama xariiqyada unugyada.
Xubin kasta oo ka mid ah shaqaalaheenna dakhliga waxqabadka ballaaran waxay qiimeeyaan shuruudaha macaamiisha iyo xiriirka ururka ee Warshada ugu iibinta wanaagsan Proteinase K CAS39450-01-6Iyada oo leh Heer Sare oo Soo-gaadhis ah iyo Taariikhda Wax-soo-saarka ee ugu Dambeeyay, Hadda, waxaan rajeyneynaa xitaa wadashaqeyn ballaaran oo lala yeesho macaamiisha dibedda ee ku saleysan faa'iidooyinka wadaagga ah.Hubi inaad dareento wax kharash ah oo aad nagala soo xidhiidho wixii macluumaad dheeraad ah.
Warshada ugu iibinta fiicanShiinaha 39450-01-6Waxaan si diiran u soo dhaweyneynaa mas'uuliyaddaada waxaanan ugu adeegi doonaa macaamiisheena gudaha iyo dibadda labadaba alaab tayo sare leh iyo adeeg heer sare ah oo loogu talagalay isbeddelka horumarinta dheeraadka ah sida had iyo jeer.Waxaan aaminsanahay inaad ka faa'iidaysan doonto xirfadeena dhawaan.
