warshadda qiimaha jaban ee Alkaline ProteinaseK CAS 39450-01-6
Waxaa naga go'an inaan bixino qiimaha tartanka, alaabooyin gaar ah iyo xalal tayo sare leh, sidoo kale si dhakhso leh u gaarsiinta warshadda qiimaha jaban ee alkalineProteinaseK CAS 39450-01-6Waxaan si daacad ah u rabnay inaan la shaqeyno macaamiisha meel kasta oo adduunka oo dhan ah. Waxaan aaminsanahay inaan ku qancin doono. Waxaan sidoo kale si diiran u soo dhawaynaynaa iibsadayaasha inay aadaan ururkayaga oo ay iibsadaan alaabtayada.
Waxaa naga go'an inaan bixino qiimaha tartanka, alaabooyin gaar ah iyo xalal tayo sare leh, sidoo kale sida ugu dhakhsaha badan ee gaarsiintaShiinaha Alkaline ProteinaseK iyo 39450-01-6, Iyada oo ay sabab u tahay tayada wanaagsan iyo qiimaha macquulka ah, badeecadaheena waxaa loo dhoofiyay in ka badan 10 dal iyo gobol. Waxa aanu rajaynaynaa in aanu la shaqayno dhamaan macaamiisha dalka iyo dibadaba. Waxaa intaa dheer, ku qanacsanaanta macaamiishu waa raadinta weligeed ah.
| No.1 dareere | |
| Waxqabadka gaarka ah ee borotiinka>800U/ml, xooga saaridu waa 20mg/mL, ma jiro hadhaaga Nickase | |
| No.2 budo | |
| Muuqashada | Budada lyophilized cad |
| Nadiifnimada Electrophoretic | ≥95% |
| Dhaqdhaqaaqa enzyme | ≥30U/mg |
| Haraaga aashitada nucleic | Aan la arki karin |
| Hadhaaga Deoxyribonuclease | Aan la arki karin |
| Haraaga Ribonuclease | Aan la arki karin |
| Gaariga No. | G1205-10ML |
| Kaydinta | Marka lagu kaydiyo -20 °C, alaabtu waxay sii haynaysaa dhaqdhaqaaqa ugu yaraan 1 sano, gaadiidka bacda. |
Proteinase Kwaa serine protease ka tirsan qoyska subtilisin oo leh firfircooni firfircoon oo enzymatic ah iyo gaar ahaan substrate-ka ballaadhan, kaas oo doorbidaya kala-goynta ester bonds iyo peptides ee u dhow C-terminus ee acids amino acids, sulfur-ka kooban amino acids, iyo bonds amino acids udgoon, kuwaas oo ah inta badan loo isticmaalo in lagu dhimo borotiinka si loo soo saaro peptides gaaban. Proteinase K waxay leedahay sifada triad catalytic-ka caadiga ah Asp39-His69-Ser224 oo u gaar ah proteases serine waxayna leedahay laba goobood oo Ca2+ ah oo ku wareegsan xarunta firfircoon si ay u kordhiso xasilloonideeda oo ay u ilaaliso dhaqdhaqaaqa enzymatic sare ee hoos yimaada xaalado kala duwan.
1. Xirmada ogaanshaha hidda-socodka.
2. Qalabka soo saarista RNA iyo DNA.
3. Ka soo saar qaybaha aan borotiinka ahayn ee unugyada waxayna hoos u dhigaan wasakhda borotiinka (sida diyaarinta tallaalada DNA-da iyo heparin).
4. Diyaarinta Chromosomal DNA-da ee Pulse Electrophoresis.
5. Burburinta reer galbeedka.
6. Horumarinta iyo soo saarista enzymatic glycated albumin reagents ee berrinkii ogaanshaha in vitro.
1. 30mg/dhalo 1g/dhalo ama 100g/dhalo
2. 1kgs/bacda biilka
Proteinase K waa serine protease deggan oo leh substrate gaar ah. Waxay hoos u dhigtaa borotiinno badan oo ku jira gobolka hooyo xitaa marka ay joogaan saabuunta. Laguma hawlgeliyo ion bir ah, wakiilada chelating (tusaale, EDTA), sulfhydryl reagents, ama tripsin ama chymotrypsin inhibitors. Waxay ku xasilloon tahay pH ballaaran oo kala duwan (4-12.5), oo leh firfircooni ugu fiican pH 6.5-9.5. Dhaqdhaqaaqa waxa lagu kicin karaa marka lagu daro wakiilada ilkaha (SDS iyo Urea). Denaturation-ka degdega ah ee ensaymku wuxuu ku dhacaa heerkul ka sarreeya 70 ° C. Autolysis of ensaymku waxay ku sii kordheysaa pH alkaline. Si kastaba ha ahaatee, Proteinase K si buuxda uma hawlgelin autolysis. Qaar ka mid ah jajabyada enzymes waxay sii wadaan inay sii wadaan dhaqdhaqaaqooda borotiinka oo dhammaystiran, xitaa ka dib markii si ballaaran loo sameeyo. Proteinase K waxaa inta badan loo isticmaalaa codsiyada bayooloojiyada molecular si loo dheefshiido borotiinnada aan loo baahnayn, sida nucleasesyada DNA ama diyaarinta RNA ee unugyada yaryar, unugyada dhaqanka, iyo dhirta. Enzyme waxaa sida caadiga ah loo isticmaalaa 50-200 ug/ml ee diyaarinta nucleic acid ee pH 7.5-8.0 iyo 37 ~ 55oC. Waqtiyada soo-kabashada waxay ku kala duwan yihiin 30 daqiiqo ilaa 18 saacadood.
Serine protease deggan oo aad u firfircoon; loo isticmaalo go'doominta borotiinka iyo aashitada nukliyeerka.Proteinase K waxaa loo isticmaalaa sifaynta RNA iyo DNA ee unugyada ama xariiqyada unugyada.
Waxaa naga go'an inaan bixino heerka tartanka, alaabooyin gaar ah iyo xalal tayo sare leh, sidoo kale sida ugu dhakhsaha badan ee gaarsiinta warshadda qiimaha jaban ee Alkaline ProteinaseKCAS39450-01-6Waxaan si daacad ah u rabnay inaan la shaqeyno macaamiisha meel kasta oo adduunka oo dhan ah. Waxaan aaminsanahay inaan ku qancin doono. Waxaan sidoo kale si diiran u soo dhawaynaynaa iibsadayaasha inay aadaan ururkayaga oo ay iibsadaan alaabtayada.
warshad qiimo jabanShiinaha Alkaline ProteinaseK iyo 39450-01-6, Iyada oo ay sabab u tahay tayada wanaagsan iyo qiimaha macquulka ah, badeecadaheena waxaa loo dhoofiyay in ka badan 10 dal iyo gobol. Waxa aanu rajaynaynaa in aanu la shaqayno dhamaan macaamiisha dalka iyo dibadaba. Waxaa intaa dheer, ku qanacsanaanta macaamiishu waa raadinta weligeed ah.
