Warshadda qiimaha jaban ee Alkaline ProteinaseK CAS 39450-01-6
Waxaan ka go'an nahay inaan bixinno qiimaha tartanka, alaabada gaarka ah iyo xalalka tayo sare leh, sidoo kale gaarsiin degdeg ah oo loogu talagalay qiimaha hoose ee warshadda AlkalineProteinaseK CAS 39450-01-6Waxaan si dhab ah u dooneynay inaan la shaqeyno macaamiisha meel kasta oo adduunka ah. Waxaan aaminsanahay inaan ku qancin doonno. Waxaan sidoo kale si diiran u soo dhaweyneynaa iibsadayaasha inay tagaan ururkayaga oo ay iibsadaan alaabtayada.
Waxaan ka go'an nahay inaan bixinno qiimaha tartanka, alaabada gaarka ah iyo xalalka tayo sare leh, sidoo kale gaarsiin degdeg ahShiinaha Alkaline ProteinaseK iyo 39450-01-6Tayada wanaagsan iyo qiimaha macquulka ah awgeed, alaabtayada waxaa loo dhoofiyay in ka badan 10 waddan iyo gobol. Waxaan rajeyneynaa inaan la shaqeyno dhammaan macaamiisha gudaha iyo dibadda. Intaa waxaa dheer, qanacsanaanta macaamiishu waa raadintayada weligeed ah.
| Dareere No.1 ah | |
| Hawlgal gaar ah oo borotiin ah> 800U/mL, fiirsashada waa 20mg/mL, ma jiraan haraaga Nickase | |
| Budada No.2 | |
| Muuqaalka | Budada cad ee lyophilized |
| Nadiifnimo Elektroforetik ah | ≥95% |
| Dhaqdhaqaaqa enzyme-ka | ≥30U/mg |
| Hadhaaga aashitada nukliyeerku | Aan la arki karin |
| Haraaga Deoxyribonuclease | Aan la arki karin |
| Haraaga Ribonuclease | Aan la arki karin |
| Lambarka Gaariga | G1205-10ML |
| Kaydinta | Marka lagu kaydiyo -20 °C, badeecadu waxay sii haynaysaa firfircooni ugu yaraan 1 sano, iyadoo la qaadayo bacaha barafka. |
Borotiinase Kwaa borotiin serine ah oo ka tirsan qoyska subtilisin oo leh firfircooni enzymatic oo hufan iyo gaar ahaansho ballaaran oo substrate ah, kaas oo si doorbid leh u burburiya xidhmooyinka ester iyo peptides ee ku xiga C-terminus ee amino acids hydrophobic, amino acids-ka ku jira sulfur, iyo xidhmooyinka amino acids aromatic, kuwaas oo inta badan loo isticmaalo in lagu burburiyo borotiinnada si loo soo saaro peptides gaagaaban. Proteinase K wuxuu leeyahay astaamaha saddex-geesoodka catalytic ee caadiga ah Asp39-His69-Ser224 oo u gaar ah proteases-ka serine wuxuuna leeyahay laba goobood oo isku xidha Ca2+ oo ku wareegsan xarunta firfircoon si loo kordhiyo xasilloonideeda loona ilaaliyo firfircoonida enzymatic sare iyadoo la raacayo xaalado kala duwan.
1. Qalabka ogaanshaha hidde-sidaha.
2. Qalabka soo saarista RNA iyo DNA-da.
3. Soo saar qaybaha aan borotiinka lahayn ee unugyada oo baabi'iya wasakhda borotiinka (sida diyaarinta tallaallada DNA-da iyo heparin).
4. Diyaarinta DNA-da Kromosomal-ka ee loogu talagalay Koronto-dhaliyaha Wadnaha.
5. Goynta reer galbeedka.
6. Horumarinta iyo soo saarista reagents-ka albumin-ka enzymatic glycated ee ku jira cilmiga ogaanshaha in vitro.
1. 30mg/dhalo 1g/dhalo ama 100g/dhalo
2. 1kgs/bac bir ah
Proteinase K waa protease serine ah oo deggan oo leh gaar ahaansho ballaaran oo substrate ah. Waxay burburisaa borotiinno badan oo ku jira xaaladda asalka ah xitaa marka ay joogaan saabuunta. Laguma dhaqaajiyo ion-yada birta ah, wakiilada chelating (tusaale ahaan, EDTA), reagents-ka sulfhydryl, ama tripsin ama chymotrypsin inhibitors. Waa mid deggan oo ka sarreeya heerka pH ballaaran (4-12.5), iyadoo leh firfircooni ugu wanaagsan pH 6.5-9.5. Hawlaha waxaa lagu kicin karaa iyadoo lagu daro wakiilada denature (SDS iyo Urea). Denature degdeg ah ee ensaymka waxay dhacdaa heerkulka ka sarreeya 70°C. Autolysis ee ensaymka ayaa si isa soo taraysa u dhacda pH-ka alkaline. Si kastaba ha ahaatee, Proteinase K si buuxda uma hawlgasho autolysis. Qaybo ka mid ah jajabyada ensaymka ayaa sii wada inay ilaaliyaan dhaqdhaqaaqooda proteolytic oo dhammaystiran, xitaa ka dib autolysis ballaaran. Proteinase K waxaa si joogto ah loogu isticmaalaa codsiyada bayoolojiga molecular-ka si loo dheefshiido borotiinnada aan loo baahnayn, sida nucleases-ka DNA-da ama diyaarinta RNA ee ka imanaya noolaha yaryar, unugyada la beeray, iyo dhirta. Ensaymka waxaa caadi ahaan loo isticmaalaa 50–200 ug/ml diyaarinta aashitada nukliyeer-ka ee pH 7.5–8.0 iyo 37~55oC. Waqtiyada ku-shubista way kala duwan yihiin 30 daqiiqo ilaa 18 saacadood.
Protease serine ah oo deggan oo aad u falcelinaya; oo loo isticmaalo kala-soocidda borotiinka iyo aashitada nucleic-ga. Proteinase K waxaa loo isticmaalaa nadiifinta RNA iyo DNA-da unugyada ama khadadka unugyada.
Waxaan ka go'an nahay inaan bixinno qiimaha tartanka, alaabada gaarka ah iyo xalalka tayo sare leh, sidoo kale gaarsiin degdeg ah oo loogu talagalay qiimaha hoose ee warshadda Alkaline ProteinaseKCAS39450-01-6Waxaan si dhab ah u dooneynay inaan la shaqeyno macaamiisha meel kasta oo adduunka ah. Waxaan aaminsanahay inaan ku qancin doonno. Waxaan sidoo kale si diiran u soo dhaweyneynaa iibsadayaasha inay tagaan ururkayaga oo ay iibsadaan alaabtayada.
Warshadda qiimaha jabanShiinaha Alkaline ProteinaseK iyo 39450-01-6Tayada wanaagsan iyo qiimaha macquulka ah awgeed, alaabtayada waxaa loo dhoofiyay in ka badan 10 waddan iyo gobol. Waxaan rajeyneynaa inaan la shaqeyno dhammaan macaamiisha gudaha iyo dibadda. Intaa waxaa dheer, qanacsanaanta macaamiishu waa raadintayada weligeed ah.
